Difference between revisions of "Part:BBa K4235030"
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===Characterization and results=== | ===Characterization and results=== | ||
| − | We successfully cloned our insert <partinfo>BBa_K4235011</partinfo> into the pET His6 vector <partinfo>BBa_K4235016</partinfo> using Ligation independent cloning. Please refer the PROS1 insert page <partinfo>BBa_K4235011</partinfo> for results. | + | We successfully cloned our insert <partinfo>BBa_K4235011</partinfo> into the pET His6 vector <partinfo>BBa_K4235016</partinfo> using Ligation independent cloning. Please refer the PROS1 insert page <partinfo>BBa_K4235011</partinfo> for results. <br> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 23:18, 11 October 2022
PROS1 expression cassette
This is the entire expression circuit for the Human protein S gene.
Characterization and results
We successfully cloned our insert BBa_K4235011 into the pET His6 vector BBa_K4235016 using Ligation independent cloning. Please refer the PROS1 insert page BBa_K4235011 for results.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1989
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1159
- 1000COMPATIBLE WITH RFC[1000]