Difference between revisions of "Part:BBa K4201005"

Revision as of 23:14, 11 October 2022

Cyto-TDS2

Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the yew tree (genus Taxus). We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast¹, which would require us to add export tags and make our engineering goal more difficult to reach. Taxadiene Synthase II⁸, a variation of taxadiene synthase from the Chinese yew has been found to have the highest output of paclitaxel out of any plant model, and is more effective in paclitaxel biosynthesis then the more well-known, Pacific Yew-derived taxadiene synthase³.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 898
Illegal BglII site found at 2211
Illegal XhoI site found at 488
Illegal XhoI site found at 755
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2311

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 <partinfo>BBa_K4201005 short</partinfo>
-Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the yew tree (genus Taxus). We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast<sup>1</sup>, which would require us to add export tags and make our engineering goal more difficult to reach. Taxadiene Synthase II<sup>8</sup>, a variation of taxadiene synthase from the Chinese yew has been found to have the highest output of paclitaxel out of any plant model, and is more effective in paclitaxel biosynthesis then the more well-known, Pacific Yew-derived taxadiene synthase<sup>3</sup>.
+Taxadiene synthase is the enzyme which makes the precursor to paclitaxel, taxadiene, from GGPP. It is naturally occurring, found in the yew tree (genus <i>Taxus</i>). We specify that this taxadiene synthase is cytosolic rather than from the MEP pathway in the chloroplast<sup>1</sup>, which would require us to add export tags and make our engineering goal more difficult to reach. Taxadiene Synthase II<sup>8</sup>, a variation of taxadiene synthase from the Chinese yew has been found to have the highest output of paclitaxel out of any plant model, and is more effective in paclitaxel biosynthesis then the more well-known, Pacific Yew-derived taxadiene synthase<sup>3</sup>.
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