Difference between revisions of "Part:BBa K4229009"

Revision as of 22:53, 11 October 2022

SnoopCatcher

The SnoopTag/Catcher system derives from a pilin of the bacterium Streptococcus pneumoniae. This system works similarly to the SpyTag/Catcher, also forming a covalent bond between a lysine and an asparagine and being constituted by short amino acid sequences. However, here the lysine can be found in the tag and the asparagine in the catcher.

Since both SnoopTag and SnoopCatcher are made of canonical amino acids, they can be linked to proteins of interest of different organisms. In addition, they bind to each other extremely specifically and the covalent bond is very stable under different pH and temperature conditions.

Via a small linker the SnoopCatcher can be added at the C- or N-terminal to any gene of interest.

In our project, we fused the SnoopCatcher to TnaA (BioBrick BBa_K4229011) for targeting to a microcompartment (wiffleball).

References [1] B. Zakeri et al., “Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin,” Proc. Natl. Acad. Sci. U. S. A., vol. 109, no. 12, 2012, doi: 10.1073/pnas.1115485109.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 130
Illegal AgeI site found at 216
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
-The SnoopCatcher and SnoopTag is a system, which was developed from S. pneumoniae pilin, which makes it possible to link Proteins with each other. In our project, we linked the T1 protein of the wiffelballs with the snoopTag to TnaA, of our Indigopathway. TnaA is fused with the SnoopCatcher as you can see in BioBrick BBa_K4229011.
+The SnoopTag/Catcher system derives from a pilin of the bacterium <i>Streptococcus pneumoniae</i>. This system works similarly to the SpyTag/Catcher, also forming a covalent bond between a lysine and an asparagine and being constituted by short amino acid sequences. However, here the lysine can be found in the tag and the asparagine in the catcher.
+Since both SnoopTag and SnoopCatcher are made of canonical amino acids, they can be linked to proteins of interest of different organisms. In addition, they bind to each other extremely specifically and the covalent bond is very stable under different pH and temperature conditions.
+Via a small linker the SnoopCatcher can be added at the C- or N-terminal to any gene of interest.
+In our project, we fused the SnoopCatcher to TnaA (BioBrick BBa_K4229011) for targeting to a microcompartment (wiffleball).
+<b>References</b>
+[1] B. Zakeri et al., “Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin,” Proc. Natl. Acad. Sci. U. S. A., vol. 109, no. 12, 2012, doi: 10.1073/pnas.1115485109.
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