Difference between revisions of "Part:BBa K4201002"

 
 
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<partinfo>BBa_K4201002 short</partinfo>
 
<partinfo>BBa_K4201002 short</partinfo>
  
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description
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Geranylgeranyl diphosphate synthase is an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized<sup>1</sup>. Geranylgeranyl diphosphate synthase converts FPP into GGPP, an important precursor in the MVA pathway. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring the addition of export tags.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 22:15, 11 October 2022


gm.cytoGGPPS

Geranylgeranyl diphosphate synthase is an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized1. Geranylgeranyl diphosphate synthase converts FPP into GGPP, an important precursor in the MVA pathway. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring the addition of export tags.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 784
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 784
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 784
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 784
    Illegal NgoMIV site found at 198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 971
    Illegal SapI.rc site found at 768