Difference between revisions of "Part:BBa K4439007"

Line 51: Line 51:
 
=====Procedure=====
 
=====Procedure=====
  
*Step 1: Prepare Buffers
+
*Step 1: <Prepare Buffers>
  
 
**Wash Buffer A (1L)
 
**Wash Buffer A (1L)

Revision as of 22:14, 11 October 2022

mSA-N[AS]4C-CBD-10xHis


Abstract

To complete

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Protein's Characterization

Description

To complete

Usage and Biology

To complete

Modeling

To complete

Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials
  • 2.5M imidazole stock solution
  • 5M NaCl solution
  • Protease inhibitor tablet
  • Turbonuclease
  • Sonicator
  • 1M HEPES
  • HisPur Ni-NTA beads in 20% ethanol
  • Disposable plastic column
  • Centrifuge going to 20 000 xg
  • 0.30 µm filters
  • Tube of bacterial culture induced with IPTG
Procedure
  • Step 1: <Prepare Buffers>
    • Wash Buffer A (1L)
      • 300 mM NaCl
      • 20 mM HEPES pH 7.5
      • 20 mM imidazole (for non specific binding)



Lab's Tips and Tricks

To complete

Results

Expression

To complete

Purification

To complete

Biofilm Fabrication

To complete

Hydrophobicity tests

To complete

References