Difference between revisions of "Part:BBa K4201001"

Latest revision as of 22:11, 11 October 2022

Tc.cytoGGPPS

Geranylgeranyl diphosphate synthase is an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway¹. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring us to add export tags. The amino acid sequence for this protein was run through a codon optimization program provided for maximum efficacy in soybeans.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 961
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 961
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 42
Illegal BglII site found at 971
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 961
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 961
Illegal NgoMIV site found at 372
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1148

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 <partinfo>BBa_K4201001 short</partinfo>
-description
+Geranylgeranyl diphosphate synthase is an essential enzyme in the MVA pathway in plants, through which paclitaxel is eventually synthesized. After GPP is converted into FPP, geranylgeranyl diphosphate synthase converts it into GGPP, an important precursor in the MVA pathway<sup>1</sup>. We are using this gene as part of our construct to ensure that paclitaxel is synthesized in the cytosol rather than through the MEP pathway in the chloroplast, which would complicate our project by requiring us to add export tags. The amino acid sequence for this protein was run through a codon optimization program provided for maximum efficacy in soybeans.
 <!-- Add more about the biology of this part here