Difference between revisions of "Part:BBa K4439007"
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====Protein Purification using Ni-NTA Beads====
 
====Protein Purification using Ni-NTA Beads====
  
−
*Materials*
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=====Materials=====
  
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. 2.5M imidazole stock solution
+
*2.5M imidazole stock solution
  
 
. 5M NaCl solution
 
. 5M NaCl solution

Revision as of 22:11, 11 October 2022

mSA-N[AS]4C-CBD-10xHis


Abstract

To complete

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Protein's Characterization

Description

To complete

Usage and Biology

To complete

Modeling

To complete

Experiments

Adapted Protocols

Protein Purification using Ni-NTA Beads

Materials
  • 2.5M imidazole stock solution

. 5M NaCl solution

. Protease inhibitor tablet

. Turbonuclease

. Sonicator

. 1M HEPES

. HisPur Ni-NTA beads in 20% ethanol

. Disposable plastic column

. Centrifuge going to 20 000 xg

. 0.30 µm filters

. Tube of bacterial culture induced with IPTG



Lab's Tips and Tricks

To complete

Results

Expression

To complete

Purification

To complete

Biofilm Fabrication

To complete

Hydrophobicity tests

To complete

References