Difference between revisions of "Part:BBa K4439007"
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Materials | Materials | ||
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. 2.5M imidazole stock solution | . 2.5M imidazole stock solution | ||
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. 5M NaCl solution | . 5M NaCl solution | ||
. Protease inhibitor tablet | . Protease inhibitor tablet |
Revision as of 22:10, 11 October 2022
mSA-N[AS]4C-CBD-10xHis
Contents
Abstract
To complete
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Protein's Characterization
Description
To complete
Usage and Biology
To complete
Modeling
To complete
Experiments
Adapted Protocols
Protein Purification using Ni-NTA Beads
Materials
. 2.5M imidazole stock solution
. 5M NaCl solution . Protease inhibitor tablet . Turbonuclease . Sonicator . 1M HEPES . HisPur Ni-NTA beads in 20% ethanol . Disposable plastic column . Centrifuge going to 20 000 xg . 0.30 µm filters . Tube of bacterial culture induced with IPTG
Lab's Tips and Tricks
To complete
Results
Expression
To complete
Purification
To complete
Biofilm Fabrication
To complete
Hydrophobicity tests
To complete