Difference between revisions of "Part:BBa K4165119"

Revision as of 21:08, 11 October 2022

HTRA Binding Peptide 25

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein.

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to the water-sites of PDZ domain of HTRA1 Protein with IC₅₀ of 0.16 ± 0.01 and ΔG of 8.0 kJ.mol^-1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry Lab Characterization

Modeling

Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0

cbeta_deviations	clashscore	molprobity	ramachandran_favored	ramachandran_outliers	Qmean_4	Qmean_6
0	0	2.64	0	0	-6.45183	-5.513969

                    Figure 1.: 3D Model of H1Y peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

Beuming, T., Farid, R., & Sherman, W. (2009). High‐energy water sites determine peptide binding affinity and specificity of PDZ domains. Protein Science, 18(8), 1609-1619.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4165119 short</partinfo>
-part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein.
+Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein.
 ===Usage and Biology===
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 <partinfo>BBa_K4165119 SequenceAndFeatures</partinfo>
-===Functional Parameters===
+===Dry Lab Characterization===
+<p style=" font-weight: bold; font-size:14px;"> Modeling </p>
 Got score of 4 out of 6 in our Quality assessment code after modeling by AlphaFold2.0
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 </html>
-===Modeling===
 <html>
 <p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1y.jpg" style="margin-left:200px;" alt="" width="500" /></p>