Difference between revisions of "Part:BBa K4165106"
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<partinfo>BBa_K4165106 short</partinfo>
 
<partinfo>BBa_K4165106 short</partinfo>
  
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part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
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Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein
  
 
===Usage and Biology===
 
===Usage and Biology===
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===Functional Parameters===
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===Dry Lab Characterization===
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<p style=" font-weight: bold; font-size:14px;"> Title </p>
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Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0.
 
Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0.
  
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</html>
 
</html>
  
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===Modeling===
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1l.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/htra1-bp/h1l.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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                  Figure 1.: 3D Model of H1L peptide Modelled by AlphaFold2.0 displayed in Pyomol.  
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                    Figure 1.: 3D Model of H1L peptide Modelled by AlphaFold2.0 displayed in Pymol.  
  
 
===References===
 
===References===

Revision as of 20:48, 11 October 2022


HTRA Binding Peptide 12

Part encodes for a synthetic peptide that binds to the PDZ domain of HTRA1 protein

Usage and Biology

Synthetic peptide acquired by Phage display, Binds specifically to PDZ domain of HTRA1 and HTRA3 Proteins with ΔG of –7.0 kJ.mol-1 and IC50 of 24 ± 8.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Dry Lab Characterization

Title

Got score of 5 out of 6 in our Quality assessment code after modeling by AlphaFold2.0.

cbeta_deviations clashscore molprobity ramachandran_favored ramachandran_outliers Qmean_4 Qmean_6
0 67.8 4.48 25 75 -6.45183 -5.51397 



                    Figure 1.: 3D Model of H1L peptide Modelled by AlphaFold2.0 displayed in Pymol.

References

1.Romero-Molina, S., Ruiz-Blanco, Y. B., Mieres-Perez, J., Harms, M., Münch, J., Ehrmann, M., & Sanchez-Garcia, E. (2022). PPI-Affinity: A Web Tool for the Prediction and Optimization of Protein–Peptide and Protein–Protein Binding Affinity. Journal of Proteome Research. 2.Runyon, S. T., Zhang, Y., Appleton, B. A., Sazinsky, S. L., Wu, P., Pan, B., ... & Sidhu, S. S. (2007). Structural and functional analysis of the PDZ domains of human HtrA1 and HtrA3. Protein Science, 16(11), 2454-2471.