Difference between revisions of "Part:BBa K4232011"
 
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[[File:BIT-China-parts-legumoglobin plasmid.png|200px|thumb|left|'''Figure 1.'''legumoglobin plasmid diagram]]
 
[[File:BIT-China-parts-legumoglobin plasmid.png|200px|thumb|left|'''Figure 1.'''legumoglobin plasmid diagram]]
 
[[File:BIT-China-parts-legumoglobin -difference.jpeg|200px|thumb|right|'''Figure 2.'''Difference in fluorescence intensity after addition of heme]]
 
[[File:BIT-China-parts-legumoglobin -difference.jpeg|200px|thumb|right|'''Figure 2.'''Difference in fluorescence intensity after addition of heme]]
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Latest revision as of 20:39, 11 October 2022


It is the "switch" that regulates the oxygen concentration in the rhizosphere

This is a plasmid consisting of legumoglobin and p5T7,it is used to transport oxygen within cells.The specific working principle is show in the BBa_K4232001.The specific construction of the legumoglobin is shown in Figure 1.The expression of legumoglobin is affected by heme, and the fluorescence intensity after the addition of heme is shown in Figure 2.

Figure 1.legumoglobin plasmid diagram
Figure 2.Difference in fluorescence intensity after addition of heme










Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25