Difference between revisions of "Part:BBa K4232010"

Latest revision as of 20:34, 11 October 2022

It is a widely regarded catalyst that oxidizes many substrates and reduces oxygen to water.

This part of the code is laccase. It is a reductive enzyme in E. coli. This part is useful for catalyzing intracellular oxygen with reducing substances, accelerating oxygen consumption and thus creating an intracellular hypoxic environment.The detailed principle of action of laccase is shown in BBa_K4232010. The specific construction of the laccase plasmid is shown in Figure 1. Since the expression of laccase is affected by copper ions, we added copper ions during the laccase culture process, and the specific difference in fluorescence intensity is shown in Figure 2.

figure 1.Laccase plasmid diagram

figure 2.Difference in fluorescence intensity after addition of copper ions

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 272
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 25

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K4232010 short</partinfo>
-This part of the code is laccase. It is a reductive enzyme in E. coli. This part is useful for catalyzing intracellular oxygen with reducing substances, accelerating oxygen consumption and thus creating an intracellular hypoxic environment.
+This part of the code is laccase. It is a reductive enzyme in E. coli. This part is useful for catalyzing intracellular oxygen with reducing substances, accelerating oxygen consumption and thus creating an intracellular hypoxic environment.The detailed principle of action of laccase is shown in '''BBa_K4232010'''.
+The specific construction of the laccase plasmid is shown in Figure 1.
+Since the expression of laccase is affected by copper ions, we added copper ions during the laccase culture process, and the specific difference in fluorescence intensity is shown in Figure 2.
+[[File:BIT-China-parts-Laccase plasmid.png|200px|left|thumb|'''figure 1.'''Laccase plasmid diagram]]
+[[File:BIT-China-parts-cueo-difference.jpeg|200px|right|thumb|'''figure 2.'''Difference in fluorescence intensity after addition of copper ions]]
 <!-- Add more about the biology of this part here