Difference between revisions of "Part:BBa K4144010"

Revision as of 20:24, 11 October 2022

Improved recombined SAMe transporter opPet8p fused with linker

Usage and Biology

Linkers can be classified into three groups: flexible, rigid and cleavable. Flexible linkers are generally composed of small, non-polar or polar residues such as Gly, Ser and Thr. The most common is the (Gly4Ser)n linker (Gly–Gly–Gly–Gly–Ser)n, where n indicates the number of repeats of the motif. Polyglycine linkers have also been evaluated, but the addition of a polar residue such as serine can reduce linker–protein interactions and preserve protein function. Due to their flexibility, these linkers are unstructured and thus provided limited domain separation in a previous study.

We WHU-China plan to use such linker to contact opPet8p and sfGFP in order to avoid the opPet8p membrane localization disruption by adding a sfGFP tag.

Figure. 1 Linker addition design into opPet8p::sfGFP recombined protein

Sequence and Features