Difference between revisions of "Part:BBa K4165256"

Revision as of 19:55, 11 October 2022

GST-Coh2-G4S-WWW

Long description

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
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WetLab Results

Transformation of GST COH WWW in BL-21 using pGS-21a vector

                                    Figure 1. Transformed plate of GST COH (L) WWW + pGS-21a

Transformation of GST COH WWW in DH-5 alpha using pJET vector

                                   Figure 2. Transformed plate of GST COH (L) WWW + pJET

Comparison between chemical lysis and sonication for GST COH WWW

Figure 3. This graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW

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 </html>
 Figure 3. This graph shows a significant difference between chemical lysis and sonication for GST COH WWW, after we had the results we optimized our protocol to use chemical lysis for GST COH WWW
-<p style=" font-weight: bold; font-size:14px;"> SDS PAGE of induced and non induced samples of GST COH WWW </p>
-<html>
-<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/sds-of-gst-coh-td28-rev.jpg" style="margin-left:200px;" alt="" width="500" /></p>
-</html>
-Figure 4. This figure shows the comparison between the induced and non induced samples of GST COH WWW, where well no.1 is the induced sample while well no.5 is the non induced samples showing that the induction successfully happened owing to the IPTG
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 ===Functional Parameters===
 <partinfo>BBa_K4165256 parameters</partinfo>
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