Difference between revisions of "Part:BBa K4323000:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
We optimize codon for expression in Escherichia coli
+
We optimize the codon for expression in <i>Escherichia coli</i>.  The sequence of the Aerolysin was adapted from [https://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1002135 Ioan Iacovache <i>et al</i> (2011)] (1)
  
  
Line 16: Line 16:
  
 
===References===
 
===References===
 +
 +
(1) Iacovache, I.; Degiacomi, M. T.; Pernot, L.; Ho, S.; Schiltz, M.; Dal Peraro, M.; van der Goot, F. G. Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin. PLoS Pathogens 2011, 7 (7).

Revision as of 19:54, 11 October 2022


Coding sequence for Aerolysin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
    Illegal AgeI site found at 313
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We optimize the codon for expression in Escherichia coli. The sequence of the Aerolysin was adapted from Ioan Iacovache et al (2011) (1)


Source

Aeromonas hydrophila

References

(1) Iacovache, I.; Degiacomi, M. T.; Pernot, L.; Ho, S.; Schiltz, M.; Dal Peraro, M.; van der Goot, F. G. Dual Chaperone Role of the C-Terminal Propeptide in Folding and Oligomerization of the Pore-Forming Toxin Aerolysin. PLoS Pathogens 2011, 7 (7).