Difference between revisions of "Part:BBa K4169030"
Ruixuan Zhu (Talk | contribs) |
Ruixuan Zhu (Talk | contribs) |
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<partinfo>BBa_K4169030 short</partinfo> | <partinfo>BBa_K4169030 short</partinfo> | ||
| − | In the presence of theophylline, expression of subsequent Cre recombinase is induced. | + | In the presence of theophylline, expression of subsequent Cre recombinase is induced.In addition, the promoter and RBS between the two loxP will be reversed, making it express genes in the other direction. |
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
dvxv | dvxv | ||
Revision as of 19:45, 11 October 2022
Cre-reverse loxP system:working when you need to express genes in the other direction
In the presence of theophylline, expression of subsequent Cre recombinase is induced.In addition, the promoter and RBS between the two loxP will be reversed, making it express genes in the other direction.
Usage and Biology
dvxv Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1292
Illegal NheI site found at 1315 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 454
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 39
Illegal AgeI site found at 140 - 1000COMPATIBLE WITH RFC[1000]