Difference between revisions of "Part:BBa K4165193"

Latest revision as of 19:40, 11 October 2022

Amyloid beta peptide 13 (Aβ 32-42)

This part encodes a part of the Amyloid 𝛽 fragment (32-42) which has the ability to bind to A𝛽 plaques inside the brain.

Usage and Biology

Segments of amyloid beta fibrils are widely used as a recognition sequence for amyloid beta plaques inside the brain, this is due to the homotypic interactions in the C-terminus of fibrils. This peptide starts from amino acids 32 to 42 of the fibril.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Dry lab

Modeling

The peptide was modeled by several software (Alphafold-Apptest-Pepfold) and the best model from Apptest scored 3 out of 6 according to our Quality assessment code.

                             Figure 1.: Amyloid-beta (32-42) top model visualized by Pymol.

References

1- Zhao, Y., Cai, J., Liu, Z., Li, Y., Zheng, C., Zheng, Y., ... & Liu, Y. (2018). Nanocomposites inhibit the formation, mitigate the neurotoxicity, and facilitate the removal of β-amyloid aggregates in Alzheimer’s disease mice. Nano letters, 19(2), 674-683.

@@ Line 11: / Line 11: @@
 ===<span class='h3bb'>Sequence and Features</span>===
 <partinfo>BBa_K4165193 SequenceAndFeatures</partinfo>
-===Features and codon optimize===
-This sequence is optimized for E. coli bacteria
-===Source===
-Homo sapiens Amyloid-beta precursor protein - UniProt (P05067)
 ===Dry lab===
 <p style=" font-weight: bold; font-size:14px;"> Modeling </p>
-Due to the nonavailability of a model to this peptide we modeled it in our used modeling software's and after the Running of the quality assessment, the models have been scored to get the top model.
+The peptide was modeled by several software (Alphafold-Apptest-Pepfold) and the best model from Apptest scored 3 out of 6 according to our Quality assessment code.
 <html>