Difference between revisions of "Part:BBa K4117002"

 
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<partinfo>BBa_K4117002 short</partinfo>
 
<partinfo>BBa_K4117002 short</partinfo>
  
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CYP2C9
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The gene CYP2C9 encodes the enzyme CYP2C9, which not only take part in the reaction where the Δ9-THC hydroxylates into 11-OH-Δ9-THC, but also catalyzes the oxygenization reaction from 11-OH-Δ9-THC to THC-COOH.[1]Therefore, we introduced Ompt, CYP2C9, his tag gene between the T7 promoter and T7 terminator of PET-Duet-I and made it successfully expressed.
  
 
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Revision as of 18:45, 11 October 2022


CYP2C9

The gene CYP2C9 encodes the enzyme CYP2C9, which not only take part in the reaction where the Δ9-THC hydroxylates into 11-OH-Δ9-THC, but also catalyzes the oxygenization reaction from 11-OH-Δ9-THC to THC-COOH.[1]Therefore, we introduced Ompt, CYP2C9, his tag gene between the T7 promoter and T7 terminator of PET-Duet-I and made it successfully expressed.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
    Illegal NotI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1386
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]