Difference between revisions of "Part:BBa K4218004"
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===Characterization===
 
===Characterization===
−
<p>A previous study indicated exons of MAP3K7 were skipped in MDS patients. In order to detect the dysregulation of RNA splicing, we designed two exon-skipping LUC reporters (MAP3K7-LUC) which contains parts of the MAP3K7 intronic and exonic sequences in the luciferase gene (more details about the principle were listed in the project description and the working model was listed below).</p >
 
 
[[File: Reporter_system.png|600px|thumb|center|]]  
 
[[File: Reporter_system.png|600px|thumb|center|]]  
  

Revision as of 18:27, 11 October 2022


The dual luciferase reporter system

According to previous studies, we found that exons of mitogen-activated protein kinase kinase kinase 7 (MAP3K7) was skipped in MDS patients. This mechanism gives rise to our idea of constructing the sensor based on the exon skipping of MAP3K7 to the plasmid containing reporter genes, which monitor the alteration of RNA splicing in cells. Our project will help us to evaluate the effectiveness of these sensors in diagnosing MDS patients in the future.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2459
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 3661