Difference between revisions of "Part:BBa K4274000"

 
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<partinfo>BBa_K4274000 short</partinfo>
  
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<i>Cl</i>SS_S533A
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===Usage and Biology===
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<i>Cl</i>SSS533A is an optimized biobrick part encoding the gene for alpha-santalene synthase from <i>Clausena lansium</i>. The enzyme catalyzes the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the alpha-santelene in a single step. It is reported that ClSS's basic amino acid residue S533’s mutation to typical nonpolar amino acid Ala could result in a 1.7-fold increase in production of alpha-santalene compared to the nonmutated strain (Jia Z.et al., 2022) .
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<br>
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This year, we are using ClSSS533A to construct composite parts ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20-B0015 (part: BBa_K4274020), ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20F96W-B0015 (part: BBa_K4274021) and ptac-RiboJ-B0034-ClSSS533A-FL-ERG20F96W-B0015 (part: BBa_K4274023). This will allow engineering E.coli DH5a (tnaA-) to heterologously express ClSSS533A. Other teams can utilize this part for E. coli alpha-santalene production.
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==Source==
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<i>Clausena lansium</i>
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==Characterization==
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Test
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<!-- -->
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4274000 SequenceAndFeatures</partinfo>
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<!--Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K4274000 parameters</partinfo>

Revision as of 18:04, 11 October 2022

ClSS_S533A

ClSS_S533A

Usage and Biology

ClSSS533A is an optimized biobrick part encoding the gene for alpha-santalene synthase from Clausena lansium. The enzyme catalyzes the conversion of the common isoprenoid intermediate farnesyl pyrophosphate (FPP) into the alpha-santelene in a single step. It is reported that ClSS's basic amino acid residue S533’s mutation to typical nonpolar amino acid Ala could result in a 1.7-fold increase in production of alpha-santalene compared to the nonmutated strain (Jia Z.et al., 2022) .
This year, we are using ClSSS533A to construct composite parts ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20-B0015 (part: BBa_K4274020), ptac-RiboJ-B0034-ClSSS533A-B0034-ERG20F96W-B0015 (part: BBa_K4274021) and ptac-RiboJ-B0034-ClSSS533A-FL-ERG20F96W-B0015 (part: BBa_K4274023). This will allow engineering E.coli DH5a (tnaA-) to heterologously express ClSSS533A. Other teams can utilize this part for E. coli alpha-santalene production.

Source

Clausena lansium

Characterization

Test

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 183
  • 1000
    COMPATIBLE WITH RFC[1000]