Difference between revisions of "Part:BBa K4335002:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022). A highly photostable and bright green fluorescent protein. Nat Biotechnol 40, 1132-1142.
 
  
 
===References===
 
===References===
 +
Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022). A highly photostable and bright green fluorescent protein. Nat Biotechnol 40, 1132-1142.

Latest revision as of 17:52, 11 October 2022


StayGold, a newly discoverd green fluorescent protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 540
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make it easier to heterologously express in Chlamydomonas reinhardtii, we have optimized the codon and added a segment of the intron.


Source

References

Hirano, M., Ando, R., Shimozono, S., Sugiyama, M., Takeda, N., Kurokawa, H., Deguchi, R., Endo, K., Haga, K., Takai-Todaka, R., et al. (2022). A highly photostable and bright green fluorescent protein. Nat Biotechnol 40, 1132-1142.