Difference between revisions of "Part:BBa K4117888:Design"
(→Design Notes) |
(→Source) |
||
Line 11: | Line 11: | ||
===Source=== | ===Source=== | ||
− | + | We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503 | |
+ | |||
+ | C-phycocyanin alpha subunit and beta subunit genes come from Part:BBa_K4117779. | ||
+ | |||
+ | PmrC promoter comes from Part:BBa_K1459006. | ||
+ | |||
+ | PmrA gene comes from Part:BBa_K3611003. | ||
+ | |||
+ | OmpT signal peptide gene comes from Part:BBa_K3805532. | ||
+ | |||
+ | OmpA signal peptide gene comes from Part:BBa_K103006. | ||
+ | |||
+ | T7 terminator gene comes from Part:BBa_B0012. | ||
+ | |||
+ | rrnB T1 Terminator gene comes from BBa_K3257020. | ||
===References=== | ===References=== |
Revision as of 17:47, 11 October 2022
UGT1A3
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 4599
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 4599
Illegal BglII site found at 4257
Illegal BamHI site found at 2887
Illegal BamHI site found at 3444 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 4599
Illegal XbaI site found at 693
Illegal SpeI site found at 713
Illegal PstI site found at 1214
Illegal AgeI site found at 3031 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 732
Design Notes
To degrade Δ9-THC, we added a pathway to sense Δ9-THC in front of the UGT1A3 gene.
Source
We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P35503
C-phycocyanin alpha subunit and beta subunit genes come from Part:BBa_K4117779.
PmrC promoter comes from Part:BBa_K1459006.
PmrA gene comes from Part:BBa_K3611003.
OmpT signal peptide gene comes from Part:BBa_K3805532.
OmpA signal peptide gene comes from Part:BBa_K103006.
T7 terminator gene comes from Part:BBa_B0012.
rrnB T1 Terminator gene comes from BBa_K3257020.