Difference between revisions of "Part:BBa K4335012:Design"

 
 
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===Design Notes===
 
===Design Notes===
  
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pCrU6.3 was constructed into plasmid <a href="https://parts.igem.org/Part:BBa_K4335038">[pTX2038]</a> <a href="https://parts.igem.org/Part:BBa_K4335040">[pTX2040]</a> as the promoter of gRNA, and was successfully introduced into Chlamydomonas reinhardtii to obtain the positive transformant.
  
  
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<i>Chlamydomonas reinhardtii</i>
  
 
===References===
 
===References===
 +
[1]Jakab, G., Mougin, A., Kis, M., Pollák, T., Antal, M., Branlant, C., and Solymosy, F. (1997). Chlamydomonas U2, U4 and U6 snRNAs. An evolutionary conserved putative third interaction between U4 and U6 snRNAs which has a counterpart in the U4atac-U6atac snRNA duplex. Biochimie 79: 387–395]<br>
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<br>[2]  Greiner, A. et al. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498–2518 (2017).

Latest revision as of 17:44, 11 October 2022


pCrU6.3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
    Illegal NotI site found at 453
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 48
    Illegal PstI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pCrU6.3 was constructed into plasmid <a href="https://parts.igem.org/Part:BBa_K4335038">[pTX2038]</a> <a href="https://parts.igem.org/Part:BBa_K4335040">[pTX2040]</a> as the promoter of gRNA, and was successfully introduced into Chlamydomonas reinhardtii to obtain the positive transformant.


Source

Chlamydomonas reinhardtii

References

[1]Jakab, G., Mougin, A., Kis, M., Pollák, T., Antal, M., Branlant, C., and Solymosy, F. (1997). Chlamydomonas U2, U4 and U6 snRNAs. An evolutionary conserved putative third interaction between U4 and U6 snRNAs which has a counterpart in the U4atac-U6atac snRNA duplex. Biochimie 79: 387–395]

[2] Greiner, A. et al. Targeting of Photoreceptor Genes in Chlamydomonas reinhardtii via Zinc-Finger Nucleases and CRISPR/Cas9. Plant Cell 29, 2498–2518 (2017).