Difference between revisions of "Part:BBa K4256188:Design"

 
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===Source===
 
===Source===
  
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It comes from the combination of composite parts BBa_K4256168, BBa_K4256106, BBa_K4256133, BBa_K4256186, BBa_K4256100, BBa_K4256106, BBa_K592012, and BBa_K4256186, and the source of each part can be checked.
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It comes from the combination of composite parts BBa_I712074, BBa_K3716001, BBa_K4256133, BBa_B0012, BBa_K4256100 and BBa_K592012, and the source of each part can be checked.
  
 
===References===
 
===References===

Latest revision as of 17:41, 11 October 2022


EforRed validation system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 651
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 651
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 651
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 651
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 651
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The use of eforRed for chromoprotein is a part borrowed from other iGEM teams; BglII is added before and HindIII is added afterwards to facilitate subsequent vector linkage and enzyme identification for recovery


Source

It comes from the combination of composite parts BBa_I712074, BBa_K3716001, BBa_K4256133, BBa_B0012, BBa_K4256100 and BBa_K592012, and the source of each part can be checked.

References