Difference between revisions of "Part:BBa K4245209"

Revision as of 17:35, 11 October 2022

hsa-mir-451a RCA Padlock Probe

This part is the sequence for the hsa-miR-451a RCA Padlock Probe, which our team designed to be specific to BBa_K4245012, hsa-miR-451a. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (BBa_K4245134/ BBa_K4245135) and the FAM and BHQ1 labeled linear probes (BBa_K4245130/BBa_K4245132) bind) and the 3’ (BBa_K4245100) and 5’(BBa_K4245107) arms of the padlock probe.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 17:35, 11 October 2022 (view source) Michellejing (Talk \| contribs) ← Older edit		Revision as of 17:35, 11 October 2022 (view source) Michellejing (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K4245209 short</partinfo>		<partinfo>BBa_K4245209 short</partinfo>

−	This part is the sequence for the hsa-miR-451a RCA Padlock Probe, which our team designed to be specific to BBa_K4245012, hsa-miR-451a. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (<partinfo>BBa_K4245134</partinfo>/ <partinfo>BBa_K4245135</partinfo>) and the FAM and BHQ1 labeled linear probes (<partinfo>BBa_K4245130</partinfo>/<partinfo>BBa_K4245132</partinfo>) bind) and the 3’ (<partinfo>BBa_K4245100</partinfo>) and 5’(<partinfo>BBa_K4245107</partinfo>) arms of the padlock probe.	+	This part is the sequence for the hsa-miR-451a RCA Padlock Probe, which our team designed to be specific to <partinfo>BBa_K4245012</partinfo>, hsa-miR-451a. This miRNA acts as a biomarker for Coronary Artery Disease, and is therefore potentially useful for the early detection of this condition. When using the Rolling Circle Amplification approach, the produced transcript contains repeats of the middle sequence (to which the DNA fluorescent aptamer split lettuce (<partinfo>BBa_K4245134</partinfo>/ <partinfo>BBa_K4245135</partinfo>) and the FAM and BHQ1 labeled linear probes (<partinfo>BBa_K4245130</partinfo>/<partinfo>BBa_K4245132</partinfo>) bind) and the 3’ (<partinfo>BBa_K4245100</partinfo>) and 5’(<partinfo>BBa_K4245107</partinfo>) arms of the padlock probe.