Difference between revisions of "Part:BBa K1795024"

 
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<partinfo>BBa_K1795024 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1795024 SequenceAndFeatures</partinfo>
  
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==iGEM2022_Nanjing-China Experiment==
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[[Image:Nanjing-China-silver-CusS-RFP.jpg|400px|thumb|right|Fluorescence detection of transformed E.coli. at different concentrations of silver ions]]
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<b>Group: Nanjing-China 2022</b>
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<b>Author: Jiankai Liu</b>
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We add new documentation to this existing Part, pCusC mKate2 (BBa_K1980007), and expand its applications in silver ions indicator through paper reading and our experiment results.
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This part consists of the promoter pCusC (BBa_K1980004), a copper responsive promoter activated by the CusS/R two-component system, with a downstream RFP variant (mKate). According to the reference we found, the histidine kinase CusS of the CusR/S two-component system functions as an Ag(I)/Cu(I)-responsive sensor kinase, which is not unexpected due to the similar chemical properties of silver and copper. These findings suggest a model for activation of the histidine kinase through metal binding events in the periplasmic sensor domain. And the histidine kinase of CusS is the key to triggering downstream reactions including RFP expression.
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We also carried out the experimental study including fluorescence detection of edited E. coli at different concentrations of silver ions. The experimental results support our speculation to some extent and further experiments are needed to confirm that.
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Revision as of 17:10, 11 October 2022


KanR Operon under R0010

Provides Kanamycin Resistance to the cell under the Promoter R0010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


iGEM2022_Nanjing-China Experiment

Fluorescence detection of transformed E.coli. at different concentrations of silver ions

Group: Nanjing-China 2022
Author: Jiankai Liu

We add new documentation to this existing Part, pCusC mKate2 (BBa_K1980007), and expand its applications in silver ions indicator through paper reading and our experiment results.

This part consists of the promoter pCusC (BBa_K1980004), a copper responsive promoter activated by the CusS/R two-component system, with a downstream RFP variant (mKate). According to the reference we found, the histidine kinase CusS of the CusR/S two-component system functions as an Ag(I)/Cu(I)-responsive sensor kinase, which is not unexpected due to the similar chemical properties of silver and copper. These findings suggest a model for activation of the histidine kinase through metal binding events in the periplasmic sensor domain. And the histidine kinase of CusS is the key to triggering downstream reactions including RFP expression.

We also carried out the experimental study including fluorescence detection of edited E. coli at different concentrations of silver ions. The experimental results support our speculation to some extent and further experiments are needed to confirm that.