Difference between revisions of "Part:BBa K4228017"
Line 10: Line 10:
  
  
−
<!-- Uncomment this to enable Functional Parameter display
+
<!-- Uncomment this to enable Functional Parameter display-->
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4228017 parameters</partinfo>
 
<partinfo>BBa_K4228017 parameters</partinfo>
 
<!-- -->
 
<!-- -->

Revision as of 17:10, 11 October 2022


Brief introduction

Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first. We cloned the sequence as BamHI-XhoI inserts in the pET-32a expression vector. pET-32a is a T7 promoter vector which can propagate in Escherichia coli after appropriate induction by IPTG.The desired polypeptide can be expressed as a fusion protein with 6xHis tag at the C-terminus for simplified purification.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 339
    Illegal NheI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 339
    Illegal BamHI site found at 28
    Illegal XhoI site found at 673
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]


Functional Parameters