Difference between revisions of "Part:BBa K4165171"
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                       Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                       Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                         variation of mRNA and protein concentrations with time compared with the wet lab results.
 
                         variation of mRNA and protein concentrations with time compared with the wet lab results.
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===WetLab Results===
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<p style=" font-weight: bold; font-size:14px;"> Comparison between the mathematical model of dry lab and the wet lab results of GST COH using chemical lysis </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/tir-wet-expression/gst-coh-chem.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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Figure 2. This graph shows the correlation between the mathematical model of dry lab and the wet lab results of GST COH using chemical lysis
 +
<p style=" font-weight: bold; font-size:14px;">Comparison between the mathematical model of dry lab and the wet lab results of GST COH using sonicator  </p>
 +
<html>
 +
<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/tir-wet-expression/gst-coh2-son.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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Figure 3. This graph shows that the mathematical model of dry lab and the wet lab results of GST COH using sonicator don't correlate with each as there is a difference between the results
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<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:59, 11 October 2022


Biobrick GST - CoH2

This composite part consists of T7 promoter (BBa_K3633015), lac operator (BBa_K4165062), RBS 4 (BBa_K4165264), GST tag (BBa_K4165070), CoH2 (BBa_K4165003), and T7 terminator (BBa_K731721).

Source

Synthesized

Usage and Biology

COH protein is a part of the Snitch system, which binds to the GST tag for the purification of the COH.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1172
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 45
    Illegal SapI.rc site found at 189

Dry lab

Modeling

Model “peptide (GST_Coh2) modeled by Alphafold GST_Coh2_f66fb_unrelaxed_rank_3_model_2” Ranked first model from our 4 ones with score 5 out of 6 and values of C Beta deviation = 0 , clash score = 13.59 , molprobitey = 1.64 , Ramachandran favored = 98.31 , Ramachandran outlier = 0.56 , Q Mean_4 = 0.643, Q Mean_6 = 0.914 


                             Figure 1. The 3D structure of COH model  Visualized by Pymol.

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 


                      Figure 1. this figure shows the results from the transcription and translation code showing the 
                        variation of mRNA and protein concentrations with time compared with the wet lab results.

WetLab Results

Comparison between the mathematical model of dry lab and the wet lab results of GST COH using chemical lysis

Figure 2. This graph shows the correlation between the mathematical model of dry lab and the wet lab results of GST COH using chemical lysis

Comparison between the mathematical model of dry lab and the wet lab results of GST COH using sonicator

Figure 3. This graph shows that the mathematical model of dry lab and the wet lab results of GST COH using sonicator don't correlate with each as there is a difference between the results