Difference between revisions of "Part:BBa K4228017"
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<h1>Brief introduction</h1> | <h1>Brief introduction</h1> | ||
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<span class='h3bb'>Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first. | <span class='h3bb'>Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first. | ||
Revision as of 16:56, 11 October 2022
Brief introduction
Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first. We cloned the sequence as BamHI-XhoI inserts in the pET-32a expression vector. pET-32a is a T7 promoter vector which can propagate in Escherichia coli after appropriate induction by IPTG.The desired polypeptide can be expressed as a fusion protein with 6xHis tag at the C-terminus for simplified purification.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 339
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 339
Illegal NheI site found at 686 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 339
Illegal BamHI site found at 28
Illegal XhoI site found at 673 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 339
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 339
- 1000COMPATIBLE WITH RFC[1000]