Difference between revisions of "Part:BBa K4228017"

 
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<partinfo>BBa_K4228017 short</partinfo>
 
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<h1>Brief introduction</h1>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first.
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We cloned the sequence as BamHI-XhoI inserts in the pET-32a expression vector. pET-32a is a T7 promoter vector which can propagate in Escherichia coli after appropriate induction by IPTG.The desired polypeptide can be expressed as a fusion protein with 6xHis tag at the C-terminus for simplified purification.
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<partinfo>BBa_K4228017 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4228017 SequenceAndFeatures</partinfo>
  

Revision as of 16:55, 11 October 2022


Ecoil composite

Brief introduction


Considering that the molecular genetic technology is more mature in Escherichia coli, we chose the basic pET-32a vector to perform preliminary validation at first. We cloned the sequence as BamHI-XhoI inserts in the pET-32a expression vector. pET-32a is a T7 promoter vector which can propagate in Escherichia coli after appropriate induction by IPTG.The desired polypeptide can be expressed as a fusion protein with 6xHis tag at the C-terminus for simplified purification.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 339
    Illegal NheI site found at 686
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 339
    Illegal BamHI site found at 28
    Illegal XhoI site found at 673
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 339
  • 1000
    COMPATIBLE WITH RFC[1000]