Difference between revisions of "Part:BBa K4218003"
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==4. Summary==
 
==4. Summary==
 
In general, we can detect the methylation level of both TFPI2 and SDC2 in patients by observing the intensity of red fluorescence in the cell free system, providing more accurate diagnosis for early screening of CRC compared to last year.
 
In general, we can detect the methylation level of both TFPI2 and SDC2 in patients by observing the intensity of red fluorescence in the cell free system, providing more accurate diagnosis for early screening of CRC compared to last year.
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Revision as of 16:44, 11 October 2022


T7-double Toehold switch-mcherry

Toehold switch is a special RNA hairpin structure, which contains trigger strand, ribosome binding site, translation start codon and a report gene. Our double toehold switch sequence (BBa_BBa_K4218016) is based on an article called Toehold Switches: De-Novo-Designed Regulators of Gene Expression (Green A, et al., 2014) and the mCherry is added to the downstream of the RNA hairpin structure.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]