Difference between revisions of "Part:BBa K4472999"
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<partinfo>BBa_K4472999 short</partinfo> | <partinfo>BBa_K4472999 short</partinfo> | ||
| − | + | The first half of the ribozyme needs to be used with a ribosome binding site upstream of it and a guide RNA downstream of it. In addition the second half needs to be present in the cell as well as the target RNA to express the reporter. If you want to design your own split ribozyme you can use our software: https://2022.igem.wiki/uni-hamburg/software | |
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Latest revision as of 16:43, 11 October 2022
Ribozyme half 1
The first half of the ribozyme needs to be used with a ribosome binding site upstream of it and a guide RNA downstream of it. In addition the second half needs to be present in the cell as well as the target RNA to express the reporter. If you want to design your own split ribozyme you can use our software: https://2022.igem.wiki/uni-hamburg/software
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 10
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]