Difference between revisions of "Part:BBa K4165254"
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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                                           Figure 2. Transformed plate of GST COH + pGS-21a  
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                                           Figure 1. Transformed plate of GST COH + pGS-21a  
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH in DH-5 alpha using pJET vector </p>
 
<p style=" font-weight: bold; font-size:14px;"> Transformation of GST COH in DH-5 alpha using pJET vector </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/gst-coh-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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                                         Figure 3. Transformed plate of GST COH + pJET  
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                                         Figure 2. Transformed plate of GST COH + pJET  
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH </p>
 
<p style=" font-weight: bold; font-size:14px;"> Comparison between chemical lysis and sonication for GST COH </p>
 
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<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/gst-coh2.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
<p><img src="https://static.igem.wiki/teams/4165/wiki/data-analysis/sonication-or-chemical/sonication-or-chemical/gst-coh2.jpg" style="margin-left:200px;" alt="" width="500" /></p>
 
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Figure 4. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH  
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Figure 3. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH  
 
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<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K4165254 parameters</partinfo>
 
<partinfo>BBa_K4165254 parameters</partinfo>
 
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Revision as of 16:12, 11 October 2022


GST-Coh2

This part encodes Cohesin 2 protein tagged with GST for its purification and characterization

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85

WetLab Results

Transfotmation of GST COH in BL-21 using pGS-21a vector 

                                          Figure 1. Transformed plate of GST COH + pGS-21a

Transformation of GST COH in DH-5 alpha using pJET vector 

                                        Figure 2. Transformed plate of GST COH + pJET

Comparison between chemical lysis and sonication for GST COH

Figure 3. This graph shows a highly significant difference between the chemical lysis and sonication for GST COH, after we had this result we optimized our protocol to use sonication for GST COH