Difference between revisions of "Part:BBa K4245135"
 
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<partinfo>BBa_K4245135 short</partinfo>
 
<partinfo>BBa_K4245135 short</partinfo>
  
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This part is the right component of the split fluorescent DNA aptamer Lettuce <partinfo>BBa_K4245133</partinfo>, developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left <partinfo>BBa_K4245134</partinfo>, induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).  
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This part is the right component of the split fluorescent DNA aptamer Lettuce (<partinfo>BBa_K4245133</partinfo>), developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left (<partinfo>BBa_K4245134</partinfo>), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).  
  
  

Latest revision as of 16:00, 11 October 2022


Lettuce Right

This part is the right component of the split fluorescent DNA aptamer Lettuce (BBa_K4245133), developed by the Weill-Cornell Medical College. Lettuce right is a 26 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce left (BBa_K4245134), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).


The mechanism in which the split Lettuce aptamer works is represented in Figure 1.

Figure 1. Diagram of the split lettuce mechanism (VarnBuhler et al., 2022).


References

VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 9
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 9
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 9
  • 1000
    COMPATIBLE WITH RFC[1000]