Difference between revisions of "Part:BBa K258008"
(New page: == Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi == EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present....) |
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== Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi == | == Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi == | ||
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli | EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli | ||
| − | Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor | + | |
| + | == Sequence and Features == | ||
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| + | <br>[1]PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA | ||
| + | <br>[2]EGF: Human Epidermal Growth Factor | ||
Revision as of 01:20, 11 October 2009
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli
Sequence and Features
[1]PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA
[2]EGF: Human Epidermal Growth Factor