Difference between revisions of "Part:BBa K4245136"
 
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This part is the left component of the split fluorescent DNA aptamer Lettuce (<partinfo>BBa_K4245133</partinfo>), developed by the Weill-Cornell Medical College. Lettuce left is a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).  
 
This part is the left component of the split fluorescent DNA aptamer Lettuce (<partinfo>BBa_K4245133</partinfo>), developed by the Weill-Cornell Medical College. Lettuce left is a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (<partinfo>BBa_K4245135</partinfo>), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).  
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The mechanism in which the split Lettuce aptamer works is represented in Figure 1.
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[[File:Lettuce_Diagram.png|thumb|center|500px|<i>Figure 1. Diagram of the split lettuce mechanism (VarnBuhler et al., 2022).</i>]]
  
 
The experimentation with this version of Lettuce left was not successful for Lambert iGEM.  
 
The experimentation with this version of Lettuce left was not successful for Lambert iGEM.  

Latest revision as of 16:00, 11 October 2022


Lettuce Left

This part is the left component of the split fluorescent DNA aptamer Lettuce (BBa_K4245133), developed by the Weill-Cornell Medical College. Lettuce left is a 42 nucleotide long DNA aptamer that, when brought in close proximity to Lettuce right (BBa_K4245135), induces fluorescence of a GFP fluorophore mimic (Z)-4-(3,5-difluoro-4-hydroxybenzylidene)-2-methyl-1-(2,2,2-trifluoroethyl)-1H-imidazol-5(4 H)-one (DFHBI-1T) (VarnBuhler et al., 2022).


The mechanism in which the split Lettuce aptamer works is represented in Figure 1.

Figure 1. Diagram of the split lettuce mechanism (VarnBuhler et al., 2022).

The experimentation with this version of Lettuce left was not successful for Lambert iGEM.



References
VarnBuhler, B. S., Moon, J., Dey, S. K., Wu, J., & Jaffrey, S. R. (2022). Detection of SARS-CoV-2 RNA using a DNA aptamer mimic of Green Fluorescent Protein. ACS Chemical Biology, 17(4), 840–853. https://doi.org/10.1021/acschembio.1c00893

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]