Difference between revisions of "Part:BBa K258007"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_S04295 short</partinfo> | <partinfo>BBa_S04295 short</partinfo> | ||
| − | '''Export of recombinant proteins in Escherichia coli using ABC | + | '''Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi''' |
| − | transporter of Erwinia chrysanthemi''' | + | |
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli | EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli | ||
Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA | Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA | ||
| − | + | EGF: Human Epidermal Growth Factor | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 00:35, 11 October 2009
No part name specified with partinfo tag. Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
EGF-TliA, EGF-LARD1 fusion proteins were secreted when ABC transporter was present. E. coli harboring the PrtDEF of E. chrysanthemi secreted more fusion proteins than without ABC transporter. In fact, in enzyme activation assay(with Hitachi Spectrophotometer) we observed that although our protein -EGF- has disulfide bonds, it was excreted succesfully. In addition, when compearing with lack of ABC domain, we saw that protein excretion into medium (supernatant) is 10-fold more with ABC transporter-PrtDEF-. PrtDEF functioned well at 37°C, the optimum growth temperature for E. coli
Sequence and Features PrtDEF: ABC transporter of Erwinia chrysanthemi for PrtA EGF: Human Epidermal Growth Factor
Sequence and Features No part name specified with partinfo tag.