Difference between revisions of "Part:BBa K4165238"
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                   Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                   Figure 1. this figure shows the results from the transcription and translation code showing the  
 
                             the concentration of the protein compared with the wet lab results.
 
                             the concentration of the protein compared with the wet lab results.
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===WetLab Results===
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His UBE2V2 in BL-21 using pGS-21a vector </p>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/ube2v2-pgs.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                  Figure 2. Transformed plate of His UBE2v2 + pGS-21a
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<p style=" font-weight: bold; font-size:14px;"> Transformation of His UBE2V2 in DH-5 alpha </p>
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<html>
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<p><img src="https://static.igem.wiki/teams/4165/wiki/parts-registry/wetlab-results/ube2v2-pjet.jpg" style="margin-left:200px;" alt="" width="500" /></p>
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</html>
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                                  Figure 3. Transformed playe of His UBE2V2 + pJET
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<!-- Uncomment this to enable Functional Parameter display  
 
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Revision as of 15:46, 11 October 2022


His-UBE2V2

This biobrick consists of T7 promotor (BBa_K3633015), RBS 5 (BBa_K4165075), 6-His tag (BBa_K4165020), UBE2V2 (BBa_K4165013), T7 Terminator (BBa_K731721), The His tag was attached to the UBE2V2 coding sequence to serve in the purification using NI-NTA column.

Source

Synthesized

Usage and Biology

Ubiquitin-conjugating E2 ligase has a role in the ubiquitination cascade for protein degradation. It has been used in this project to target and degrade both tau and Aβ proteins which are both considered the main causes of Alzheimer’s Disease pathogenesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Dry lab

Mathematical modeling

Transcription rate and translation rate under T7 promotor

the mathematical modeling was based on our code for the calculation of transcription and translation (you can find it in the code section) beside with the estimated results from the wet lab. 

                  Figure 1. this figure shows the results from the transcription and translation code showing the 
                           the concentration of the protein compared with the wet lab results.