Difference between revisions of "Part:BBa K4365012"

 
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<partinfo>BBa_K4365012 short</partinfo>
 
<partinfo>BBa_K4365012 short</partinfo>
  
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HCF-3 is a highly secreted protein found in Cladosporium fulvum. It belongs to a protein family called hydrophobins. A signal peptide from such proteins should increase the secretion of a protein of interest in yeast if the signal peptide signal is placed before it.  
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HCF-3 is a protein found in <i>Cladosporium fulvum</i> and belongs to a family of highly secreted proteins called hydrophobins. The signal peptide from the protein led to no [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365020 turboRFP] secretion efficiency in ''Saccharomyces cerevisiae''.
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{| class="wikitable" style="margin-left: auto; margin-right: auto; border: none; width:600px;"
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|+ Collection of fungal signal peptides
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|-
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!
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! ID
 +
! Source
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! Species
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! Prediction coefficient <ref name="signalp">José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z </ref>
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! turboRFP secretion
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|- style="text-align:center;"
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|sp1 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365006 BBa_K4365006] || MPGI || <i>Magnaporthe grisea</i> || 0.9562 || '''Yes'''
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|- style="text-align:center;"
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|sp2 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365007 BBa_K4365007] || RodA || <i>Aspergillus nidulans</i> || 0.9553 || '''Yes'''
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|- style="text-align:center;"
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|sp3 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365008 BBa_K4365008] || HYPI  || <i>Aspergillus fumigatus</i> || 0.9703 || No
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|- style="text-align:center;"
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|sp4 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365009 BBa_K4365009] || SsgA || <i>Metarhizium anisopliae</i> || 0.9126 || No
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|- style="text-align:center;"
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|sp5 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365010 BBa_K4365010] || HCF-4 || <i>Cladosporium fulvum</i> || 0.9126 || '''Yes'''
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|- style="text-align:center;"
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|sp6 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365025 BBa_K4365025] || HCF-5 || <i>Cladosporium fulvum</i> || 0.9234 || NA
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|- style="text-align:center;"
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|sp7 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365026 BBa_K4365026] || CCG-2 || <i>Neurospora crassa</i> || 0.9566 || NA
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|- style="text-align:center;"
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|sp8 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365011 BBa_K4365011] || HCF-1 || <i>Cladosporium fulvum</i> || 0.6850 || '''Yes'''
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|- style="text-align:center;"
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|sp9 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365027 BBa_K4365027] || HCF-2 || <i>Cladosporium fulvum</i> || 0.8144 || NA
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|- style="text-align:center;"
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|sp10 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365012 BBa_K4365012] || HCF-3 || <i>Cladosporium fulvum</i> || 0.7934 || No
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|- style="text-align:center;"
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|sp11 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365013 BBa_K4365013] || SC3 || <i>Schizophyllum commune</i> || 0.6327 || No
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|- style="text-align:center;"
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|sp12 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365014 BBa_K4365014] || ABH3 || <i>Agaricus bisporus</i> || 0.9458 || '''Yes'''
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|- style="text-align:center;"
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|sp13 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365028 BBa_K4365028] || COH1 || <i>Coprinus cinereus</i> || 0.8545 || NA
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|- style="text-align:center;"
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|sp14 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365015 BBa_K4365015] || FBH1 || <i>Pleurotus ostreatus</i> || 0.3367 || '''Yes'''
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|- style="text-align:center;"
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|sp15 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365016 BBa_K4365016] || Aa-PRI2 || <i>Agrocybe aegerita</i> || 0.6356 || '''Yes'''
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|- style="text-align:center;"
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|sp16 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365033 BBa_K4365033] || Hyd-Pt1 || <i>Pisolithus tinctorius</i> || 0.8352 || NA
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|- style="text-align:center;"
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|sp17 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365017 BBa_K4365017] || HFBI || <i>Trichoderma reesei</i> || 0.9851 || '''Yes'''
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|- style="text-align:center;"
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|sp18 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365018 BBa_K4365018] || HFBII || <i>Trichoderma reesei</i> || 0.6862 || '''Yes'''
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|- style="text-align:center;"
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|sp19 || [https://parts.igem.org/wiki/index.php?title=Part:BBa_K4365019 BBa_K4365019] || α-mating factor || <i>Saccharomyces cerevisiae</i> || 0.9988 || '''Yes'''
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|}
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<references />
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<br>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4365012 SequenceAndFeatures</partinfo>
  
 
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<span class='h3bb'>Sequence and Features</span>
 
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Revision as of 15:39, 11 October 2022

Signal peptide of HCF-3 from Cladosporium fulvum

HCF-3 is a protein found in Cladosporium fulvum and belongs to a family of highly secreted proteins called hydrophobins. The signal peptide from the protein led to no turboRFP secretion efficiency in Saccharomyces cerevisiae.

Collection of fungal signal peptides
ID Source Species Prediction coefficient [1] turboRFP secretion
sp1 BBa_K4365006 MPGI Magnaporthe grisea 0.9562 Yes
sp2 BBa_K4365007 RodA Aspergillus nidulans 0.9553 Yes
sp3 BBa_K4365008 HYPI Aspergillus fumigatus 0.9703 No
sp4 BBa_K4365009 SsgA Metarhizium anisopliae 0.9126 No
sp5 BBa_K4365010 HCF-4 Cladosporium fulvum 0.9126 Yes
sp6 BBa_K4365025 HCF-5 Cladosporium fulvum 0.9234 NA
sp7 BBa_K4365026 CCG-2 Neurospora crassa 0.9566 NA
sp8 BBa_K4365011 HCF-1 Cladosporium fulvum 0.6850 Yes
sp9 BBa_K4365027 HCF-2 Cladosporium fulvum 0.8144 NA
sp10 BBa_K4365012 HCF-3 Cladosporium fulvum 0.7934 No
sp11 BBa_K4365013 SC3 Schizophyllum commune 0.6327 No
sp12 BBa_K4365014 ABH3 Agaricus bisporus 0.9458 Yes
sp13 BBa_K4365028 COH1 Coprinus cinereus 0.8545 NA
sp14 BBa_K4365015 FBH1 Pleurotus ostreatus 0.3367 Yes
sp15 BBa_K4365016 Aa-PRI2 Agrocybe aegerita 0.6356 Yes
sp16 BBa_K4365033 Hyd-Pt1 Pisolithus tinctorius 0.8352 NA
sp17 BBa_K4365017 HFBI Trichoderma reesei 0.9851 Yes
sp18 BBa_K4365018 HFBII Trichoderma reesei 0.6862 Yes
sp19 BBa_K4365019 α-mating factor Saccharomyces cerevisiae 0.9988 Yes
  1. ↑ José Juan Almagro Armenteros et al. (2019) SignalP 5.0 improves signal peptide predictions using deep neural networks Nature Biotechnology, 37, 420-423, doi: 10.1038/s41587-019-0036-z


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]