Difference between revisions of "Part:BBa K4365004"

 
 
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<partinfo>BBa_K4365004 short</partinfo>
 
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SMT3 protein is a Ubiquitin-like protein of yeast origin. It could be used as a protein tag that can be removed from the protein of interest without scarring its sequence.
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SMT3 protein is a Ubiquitin-like protein of yeast origin. The SUMO gene commonly used is a gene of yeast origin called Smt3. When a protein fused to this tag is expressed in a prokaryote like E. coli, the cells need to be lysed and then the protein is purified and treated with the SUMO protease Ulp1, which is also of yeast origin.
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The cleavage site recognized by SUMO protease is evolutionarily conserved and is located at the last two glycine residues of the SUMO tag. This is where the SUMO protease carries out a proteolytic reaction that dissociates the tag from the downstream cargo protein. It can aminoterminally fused to a protein and can be removed from it without leaving aminoacids <ref> Hickey CM, Wilson NR, Hochstrasser M. (2012) Function and regulation of SUMO proteases, Nature reviews. Molecular Cell Biology.;13(12):755-766. DOI: 10.1038/nrm3478. PMID: 23175280; PMCID: PMC3668692.</ref>.
  
 
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Latest revision as of 15:37, 11 October 2022


SMT3 (SUMO)

SMT3 protein is a Ubiquitin-like protein of yeast origin. The SUMO gene commonly used is a gene of yeast origin called Smt3. When a protein fused to this tag is expressed in a prokaryote like E. coli, the cells need to be lysed and then the protein is purified and treated with the SUMO protease Ulp1, which is also of yeast origin.

The cleavage site recognized by SUMO protease is evolutionarily conserved and is located at the last two glycine residues of the SUMO tag. This is where the SUMO protease carries out a proteolytic reaction that dissociates the tag from the downstream cargo protein. It can aminoterminally fused to a protein and can be removed from it without leaving aminoacids [1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ Hickey CM, Wilson NR, Hochstrasser M. (2012) Function and regulation of SUMO proteases, Nature reviews. Molecular Cell Biology.;13(12):755-766. DOI: 10.1038/nrm3478. PMID: 23175280; PMCID: PMC3668692.