Difference between revisions of "Part:BBa K4375013"
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A myc tag is a polypeptide protein tag derived from the c-myc gene, and we added a SpHI restriction site to its N-terminal. | A myc tag is a polypeptide protein tag derived from the c-myc gene, and we added a SpHI restriction site to its N-terminal. | ||
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| − | + | ==Sequence and Features== | |
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<partinfo>BBa_K4375013 SequenceAndFeatures</partinfo> | <partinfo>BBa_K4375013 SequenceAndFeatures</partinfo> | ||
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| + | ==References== | ||
„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/ | „Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/ | ||
Latest revision as of 15:36, 11 October 2022
Myc epitope tag with SphI restriction site
A myc tag is a polypeptide protein tag derived from the c-myc gene, and we added a SpHI restriction site to its N-terminal.
Usage and Biology
The Myc-tag, a commonly used tag in research and biological applications, is derived from the human c-myc oncogene (410-419 at the C-terminus). The Myc-tag is used in detecting the expression of recombinant proteins when it is fused to the protein of interest. Its sequence is EQKLISEEDL. It is commonly used in Western blotting, ELISA, protein purification or co-immunoprecipitation. This part contains a SpHI restriction site making it easier to be used in molecular cloning.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
„Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.” https://www.ptglab.com/news/blog/myc-tag-an-epitope-tag-for-protein-characterization-protein-interaction-analysis-and-purification/