Difference between revisions of "Part:BBa K4252023:Experience"
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| − | Enter the review information here. | + | Enter the review information here.We cultivate BL21 strain with such plasmid at 30 ℃, 33 ℃, 37 ℃ and 42 ℃, test OD600 absorbance value after culture, break the strain and test fluorescence intensity. |
| − | We cultivate BL21 strain with such plasmid at 30 ℃, 33 ℃, 37 ℃ and 42 ℃, test OD600 absorbance value after culture, break the strain and test fluorescence intensity. | + | |
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Revision as of 15:28, 11 October 2022
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_K4252023
We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed. That is, the temperature sensitive control element cI857 is controlled by temperature
User Reviews
UNIQ87c721ed465b049f-partinfo-00000000-QINU UNIQ87c721ed465b049f-partinfo-00000001-QINU