Difference between revisions of "Part:BBa K4252023:Experience"
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===Applications of BBa_K4252023=== | ===Applications of BBa_K4252023=== | ||
| + | We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed. | ||
| + | That is, the temperature sensitive control element cI857 is controlled by temperature | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 15:28, 11 October 2022
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Applications of BBa_K4252023
We cultured the plasmids transferred into cI857, phlF, R promoter and mCherry at 30℃ ,33℃ ,37℃ and 42℃ . From the fluorescence data corresponding to our temperature gradient, the red fluorescence expression was very weak in the temperature range of 30 ℃ to 34 ℃, indicating that the dimer formed by cI857 inhibited the R promoter at this time, leading to the subsequent weakening of mCherry expression; The strong red fluorescence expression at 37 ℃ to 42 ℃ indicated that cI857 dimer depolymerized and the inhibition of R promoter was relieved, and then mCherry was normally expressed. That is, the temperature sensitive control element cI857 is controlled by temperature
User Reviews
UNIQ83dd926d0bb45bfa-partinfo-00000000-QINU UNIQ83dd926d0bb45bfa-partinfo-00000001-QINU