Difference between revisions of "Part:BBa K4117022:Design"

(→‎Design Notes)
(→‎Source)
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===Source===
 
===Source===
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We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P11712
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CYP2C19 is important
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===References===
 
===References===

Revision as of 15:28, 11 October 2022


CYP2C19


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
    Illegal NotI site found at 284
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1395
    Illegal BamHI site found at 3
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
    Illegal PstI site found at 1357
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Since this subunit has been proved to be expressed successfully, we did not re-engineer this protein gene.

Source

We design the DNA sequence based on the amino acid sequence according to E·coli codon preference.The amino acid sequence is from UniProt:P11712

References