Difference between revisions of "Part:BBa K4146103"

Latest revision as of 15:21, 11 October 2022

TcCS2

TcCS2 is used for caffeine synthesis. TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine. The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.

This gene can be found in E. coli Nissle 1917

This gene is present in part <a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 549
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 TcCS2 from C. sinensis has a higher propensity to recognize 3-mX and can convert 3-mX to theobromine.
 The main caffeine biosynthetic pathway is a sequence consisting of xanthosine→7-methylxanthosine→7-methylxanthine→theobromine→caffeine. Genes encoding N-methyltransferases involved in three of these four reactions have been isolated and the molecular structure of N-methyltransferases investigated.
 This gene can be found in E. coli Nissle 1917
-This gene is present in part<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>
+This gene is present in part
+<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>.
 <!-- Add more about the biology of this part here