Difference between revisions of "Part:BBa K4365003"

 
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Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.
 
Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K4365003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K4365003 SequenceAndFeatures</partinfo>
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===Usage and Biology===
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[[File:BBa_K4365003.jpeg|400px|left|thumb|caption]]
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Revision as of 15:21, 11 October 2022


turboRFP Selection Device with constitutive promoter

Improvement of BBa_J04450 part. In the original construct, mRFP1 expression was observed irrespective of IPTG induction. So we replaced the inducible LacI-regulated promoter (BBa_R0010) with a constitutive (BBa_J23100) for colony screening.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 683


Usage and Biology

caption