Difference between revisions of "Part:BBa K4146101"
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"Full wiffelball" with tags of the BMCs under regulation of lambdapLhybrid promotor and LacI promoto. The lactose operon is a group of genes involved in lactose breakdown, consisting of repressors and manipulating sequences of the lactose system, making a set of genes related to lactose metabolism regulated synchronously. It has negative regulation mechanism: induce the inactivation of lactose: repressor protein, shedding from LacO, (+) transcription; lactose deficiency: repressor protein binds to LacO, RNA polymerase cannot bind to LacP, (-) transcription. Positive regulation mechanism: can induce activator: CAPorCRP (cAMP binding protein), CRP-cAMP and initiator gene binding, (+) transcription. | "Full wiffelball" with tags of the BMCs under regulation of lambdapLhybrid promotor and LacI promoto. The lactose operon is a group of genes involved in lactose breakdown, consisting of repressors and manipulating sequences of the lactose system, making a set of genes related to lactose metabolism regulated synchronously. It has negative regulation mechanism: induce the inactivation of lactose: repressor protein, shedding from LacO, (+) transcription; lactose deficiency: repressor protein binds to LacO, RNA polymerase cannot bind to LacP, (-) transcription. Positive regulation mechanism: can induce activator: CAPorCRP (cAMP binding protein), CRP-cAMP and initiator gene binding, (+) transcription. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | This gene can be found in E. coli Nissle 1917 | |
| + | This gene is present in part<a href = "https://parts.igem.org/Part:BBa_K4146106"> BBa_K4146106</a>, | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:15, 11 October 2022
pLlacO1
"Full wiffelball" with tags of the BMCs under regulation of lambdapLhybrid promotor and LacI promoto. The lactose operon is a group of genes involved in lactose breakdown, consisting of repressors and manipulating sequences of the lactose system, making a set of genes related to lactose metabolism regulated synchronously. It has negative regulation mechanism: induce the inactivation of lactose: repressor protein, shedding from LacO, (+) transcription; lactose deficiency: repressor protein binds to LacO, RNA polymerase cannot bind to LacP, (-) transcription. Positive regulation mechanism: can induce activator: CAPorCRP (cAMP binding protein), CRP-cAMP and initiator gene binding, (+) transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]