Difference between revisions of "Part:BBa K4365002:Design"

 
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===Design Notes===
 
===Design Notes===
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Was amplified using a forward primer containing the BsmBI restriction site and a reverse primer containing the BsrBI restriction site
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Was amplified using primers from the p426 <ref>Mumberg D, Müller R, Funk M. (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds, Gene;156(1):119-122. doi:10.1016/0378-1119(95)00037-7</ref>
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===Source===
 
===Source===

Latest revision as of 14:59, 11 October 2022


2 micron Ori


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 360
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 360
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Was amplified using primers from the p426 [1]

Source

p426 Mumberg plasmid

References

  1. ↑ Mumberg D, Müller R, Funk M. (1995) Yeast vectors for the controlled expression of heterologous proteins in different genetic backgrounds, Gene;156(1):119-122. doi:10.1016/0378-1119(95)00037-7