Difference between revisions of "Part:BBa K4204020:Design"
Jianfei Song (Talk | contribs) (→Design Notes) |
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===Source=== | ===Source=== | ||
| − | The protein quality control system is from 's work, the crystal violet induction system is from 's work. Other components are from BBa_K653003.Assembled by BNDS_China 2022. | + | The protein quality control system is from Yang et al's work [1], the crystal violet induction system is from 's work [2]. Other components are from BBa_K653003.Assembled by BNDS_China 2022. |
===References=== | ===References=== | ||
Revision as of 14:58, 11 October 2022
Crystal violet induced GFP improved with ProQC system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1166
Illegal NheI site found at 1248
Illegal NheI site found at 2332 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1045
Illegal BamHI site found at 1943 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 475
Illegal NgoMIV site found at 1995
Illegal AgeI site found at 625 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The initial design of the part has a flaw (a gap between the RBS and the start codon leads to a frameshift of the resulting protein). This problem is fixed now by another round of design, build and test.
Source
The protein quality control system is from Yang et al's work [1], the crystal violet induction system is from 's work [2]. Other components are from BBa_K653003.Assembled by BNDS_China 2022.