Difference between revisions of "Part:BBa K4365001"

(→‎Usage and Biology)
(→‎Usage and Biology)
Line 10: Line 10:
  
 
===Usage and Biology===
 
===Usage and Biology===
−
[[File:BBa_K4365001_anemone.jpg|100px|thumb|Figure 1. Picture of the sea anemone ''Entacmaea quadricolor''. Image from Wikipedia.org]]
+
[[File:BBa_K4365001_anemone.jpg|200px|thumb|Figure 1. Picture of the sea anemone ''Entacmaea quadricolor''. Image from Wikipedia.org]]
 
TurboRFP is a red-orange fluorescent protein derived from sea anemone ''Entacmaea quadricolor'' <ref name="ameno">Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062</ref>. It possesses prolonged fluorescence lifetime and high pH-stability, is fast-maturing, and is more than twice brighter than DsRed2. <ref name="ameno" />.  
 
TurboRFP is a red-orange fluorescent protein derived from sea anemone ''Entacmaea quadricolor'' <ref name="ameno">Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062</ref>. It possesses prolonged fluorescence lifetime and high pH-stability, is fast-maturing, and is more than twice brighter than DsRed2. <ref name="ameno" />.  
  
 
[[File:BBa_K4365001_spectra.png|600px|center|thumb|Figure 2. Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. 187 % brighter than of EGFP. [https://www.fpbase.org/protein/turborfp/ FP database] ]]
 
[[File:BBa_K4365001_spectra.png|600px|center|thumb|Figure 2. Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. 187 % brighter than of EGFP. [https://www.fpbase.org/protein/turborfp/ FP database] ]]
  
−
[[File:File:BBa_K4365001_bacteria.png|300px|center|thumb|Figure 3. Fluorescent bacterial colonies with mRFP1 and turboRFP1 under transilluminator.]]
+
[[File:BBa_K4365001_bacteria.png|300px|center|thumb|Figure 3. Fluorescent bacterial colonies with mRFP1 and turboRFP1 under transilluminator.]]
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 14:53, 11 October 2022


turboRFP

Turbo Red Fluorescent Protein (turboRFP) is an orange fluorescent protein isolated from Entacmaea quadricolor with excitation and emission maxima 553 and 574 respectively. It could be used as a selection marker or for protein detection if fused with another protein.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Figure 1. Picture of the sea anemone Entacmaea quadricolor. Image from Wikipedia.org

TurboRFP is a red-orange fluorescent protein derived from sea anemone Entacmaea quadricolor [1]. It possesses prolonged fluorescence lifetime and high pH-stability, is fast-maturing, and is more than twice brighter than DsRed2. [1].

Figure 2. Emission (EM) and Excitation (EX) spectra of turboRFP. Excitation maximum = 553 nm. Emission maximum = 574 nm. 187 % brighter than of EGFP. FP database
Figure 3. Fluorescent bacterial colonies with mRFP1 and turboRFP1 under transilluminator.
  1. ↑ 1.0 1.1 Merzlyak, E., Goedhart, J., Shcherbo, D. et al. Bright monomeric red fluorescent protein with an extended fluorescence lifetime. Nat Methods 4, 555–557 (2007). https://doi.org/10.1038/nmeth1062